Study results on the molecular genetic, morphometric and sexual characteristics of the beluga (Huso huso L., 1758) grown in the aquaculture of the Republic of Belarus
Azerbaijan Journal of Physiology
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Keywords

aquaculture
Beluga (Huso huso L., 1758)
Molecular Genetics
morphometric characteristics
sex determination

How to Cite

1.
Slukvin AM, Dromashko SY, Balashenko NA, Barulin NV, Barmintseva AY. Study results on the molecular genetic, morphometric and sexual characteristics of the beluga (Huso huso L., 1758) grown in the aquaculture of the Republic of Belarus. Azerb. J. Physiol. 2022;37(1):69-80. doi:10.59883/ajp.21

Abstract

The subject of the study was the genetic and phenotypic characteristics of Beluga (Huso huso (Linnaeus, 1758)) grown in the warm-water aquaculture at the “Selets” fish farm (the Republic of Belarus). Molecular-genetic analysis was performed using STR-markers and species-specific PCR revealed the absence of hybrid individuals in the analyzed broodstock of (Huso huso L.). Affiliation of individuals to the Caspian population was established based on the sequence analysis of the 367 bp region of the mitochondrial D-loop. Using six STR loci, the population structure was analyzed. The population structure and gene pool of the only broodstock of (Huso huso L.) in Belarus were compared with domesticated (H. huso L.) from the Russian Federation. The analysis showed a lack of genetic variation and the heterozygote excess in Beluga from the Belarusian farm. Analysis of morphometric indicators revealed that the conditions in which the Beluga broodstock is kept at the fish farm “Selets” should be improved. Using ultrasound scanning, the following stages of gonad maturity were established: stages II, III, and IV in males; II, II semi-fatty, II fatty, and II-III in females. The beluga broodstock at the fish farm “Selets” is mainly represented by females (the females exceeded the males by 2.3 times in terms of their number).

https://doi.org/10.59883/ajp.21
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References

Barmintseva AE, Mugue NS. [The use of microsatellite loci for identification of sturgeon species (Acipenseridae) and hybrid forms]. Genetika. 2013 Sep;49(9):1093-105. Russian. https://doi.org/10.7868/s0016675813090038.

Birstein VJ, Bemis WE, Waldman J. The threatened status of Acipenseriform species: a summary in Sturgeon Biodiversity and Conservation. Dordrecht: Kluwer Academic Publishers; 1997. pp.427-435.

Boscari E, Vitulo N, Ludwig A, Caruso C, Mugue NS, Suciu R, Onara DF, Papetti Ch, Marino IAM, Zane L, Congiu L. Fast genetic identification of the Beluga sturgeon and its sought-after caviar to stem illegal trade. Food Control. 2017;75:145-52.

Chebanov MS, Galich EV. Sturgeon hatchery manual. FAO Fisheries and Aquaculture Technical Paper 2013. pp.558, 338. ISBN 978.92.5.106823.6.

Doukakis P, Birstein VJ. and De Salle R. Intraspecific structure within three caviar producing sturgeons (Acipenser gueldenstaedtii, A. stellatus, and Huso huso) based on mitochondrial DNA analysis. J. Appl Ichthyol. 2005;21:457-460. https://dx.doi.org/10.1111/j.1439-0426.2005.00652.x

Fopp-Bayat D, Kuciński M, Liszewski T, Teodorowicz T, Łączyńska B, Lebeda I. Genetic protocol of Atlantic sturgeon Acipenser oxyrinchus (L.) fry for restocking the Vistula river, Poland. Journal of Survey in Fisheries Sciences. 2015;2(1):1-10, https://dx.doi.org/10.18331/SFS2015.2.1.1

Havelka M, Fujimoto T, Hagihara S, Adachi S, Arai K. Nuclear DNA markers for identification of Beluga and Sterlet sturgeons and their interspecific Bester hybrid. Sci Rep. 2017 May 10;7(1):1694. https://doi.org/10.1038/s41598-017-01768-3.

King TL, Lubinski BA, Spidle AP. Microsatellite DNA variation in Atlantic sturgeon (Acipenser oxyrinchus) and cross-species amplification in Acipenseridae. Conservation Genetics. 2001;2:103-119. https://doi.org/10.1023/A:1011895429669

Mugue N, Barmintseva A, Rastorguev S, Mugue V, Barmintsev V. Polymorphism of the mitochondrial DNA control region in 8 sturgeon species and development of a system for DNA-based species identification. Russ. J. Genet. 2008;44:793-798. https://doi.org/10.1134/S1022795408070065 (Russian).

Pravdin IF. The guide for fish study (with focus on freshwater fish). Moscow: Food Industry. 1966. 376 p. (In Russian).

Raymakers C. CITES, the Convention on International Trade in Endangered Species of wild fauna and flora: its role in the conservation of Acipenseriformes. J Appl Ichthyol. 2006;22(1):53-65. https://doi.org/10.1111/j.1439-0426.2007.00929.x

Red Book of Ukraine. Animal World. Akimova, I.A. (Ed.). Kiev. Global Consulting; 2009. 600 p. ISBN 978-966-97059-0-7. (In Ukrainian)

Red Data Book of the Republic of Bulgaria. Volume 2 – Animals. Golemanski, V. & al. (Eds). Sofia. Institute of Biodiversity and Ecosystem Research – BAS & Ministry of Environment and Water; 2015. 881 p. ISBN 978-954-9746-22-8.

Red Data Book of the Russian Federation (animals). Danilov-Danilyan, V.I. & al. (Eds). Moscow: AST Astrel; 2001. 862 p. ISBN 5-17-005792-X, 5-271-00651-4. (In Russian).

Sturgeons complex genetic research: genome organization, phylogeny and development of natural populations of Russian Federation recovery genetic monitoring techniques. Koltzov Institute of Developmental Biology of Russian Academy of Sciences; project head N.S. Mugue. – Moscow, 2017. № 15-29-02766 ofi_m. (In Russian).

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