Keywords
blood serum
saliva
dihydropyrimidinase-related protein 2
natural autoantibodies
cortisol
How to Cite
Abstract
The studies were carried out on patients doomed to undergo surgery. Blood samples were collected on the day of surgery from the patients’ veins into test tubes containing 5% EDTA as an anticoagulant. Platelets and serum were purified and with the application of an indirect ELISA-test, the level of dihydropyrimidinase-related protein 2 (DRP2) was evaluated in the platelets, while the level of natural anti-DRP2 autoantibodies was measured in the blood serum. Concomitantly, the level of DRP2 was estimated in the patients’ saliva sampled on the same day. The results showed upregulation of DRP2 in the platelets and natural anti-DRP2 autoantibodies in the serum of the patients. Evaluation of the level of the stress hormone cortisol in the patients’ blood serum revealed its upregulation. At the same time downregulation of DRP2 in the patients’ saliva was noticed. Based on the obtained data, we came to a conclusion that evaluation of DRP2 in the platelets, natural anti-DRP2 autoantibodies in the blood serum, and DRP2 in the saliva might serve as a valid indicator of excitation level in humans.
References
Allakhverdieva TN, Mehdiyev KS, Gaisina AA, Mekhtiev AA. Activation of the serotonergic system promotes survival of different animal species exposed to bacterial and chemical toxins. J Evol Biochem Phys. 2019, 55(1): 24-29. https://doi.org/10.1134/S0022093019010034
Guseĭnov ShB, Mekhtiev AA. [The role of serotonin-modulated anticonsolidation protein in memory formation in rats in a shuttle box]. Zh Vyssh Nerv Deiat Im I P Pavlova. 2012 Mar-Apr;62(2):208-15.
Mekhtiev AA. Detection of protein with anticonsolidation properties in the rat brain. Bull Exp Biol Med. 2000 Aug;130(8):739-42. https://doi.org/10.1007/BF02766081.
Mekhtiev AA, Movsum-zadeh SK. Antimutagenic activity of serotoninergic system and underlying mechanisms in fry of sturgeon and goldfish. J Evol Biochem Phys. 2008, 44(5):562-568. https://doi.org/10.1134/S0022093008050046
Mustafayev NJ, Mekhtiev AA. Adaptive increase of level of serotonin-modulated anticonsolidational protein in tissues of semimigratory and migratory fish at increase of water salinity. J Evol Biochem Phys 2013;49(6):608-614. https://doi.org/10.1134/S002209301306008X
Mustafayev NJ, Mekhtiev AA. Character of changes in serotonin-modulating anticonsolidation protein and cytochrome P-450 in tissues of Alburnoides bipunctatus eichwaldi caught in the rivers of Azerbaijan Republic. J Evol Biochem Phys 2014;50(3):215-220. https://doi.org/10.1134/S002209301403003X
Shapell S, Detwiler C, Boquet A, Wiegmann D. Human error and commercial aviation accidents: A comprehensive, fine-grained analysis using HFACS. Federal Aviation Administration. DOT/FAA/AM-06/18. Office of Aerospace Medicine Washington, DC 20591. 2006.
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